Essay regarding Vitamin C Content of Apple Juice

Supplement C Content material of Apple Juice


Complete lack of ascorbic chemical p (a. k. a Supplement C) in the diets of humans and other

primates leads to a classic nutritional disease, scurvy. This kind of disease was widespread in

Europe during the 15th and 16th centuries, nonetheless it is exceptional today. Ascorbic acid is usually

extensively distributed in nature, nonetheless it occurs in extremely substantial concentrations in citrus and

green plants just like green peppers and kale. Ascorbic acidity can be synthesized by almost all

plants and pets, with the exception of humans, other primates, and guinea pigs.

Therefore , vitamin C has to be present in each of our dietary substances.

The fundamental function of ascorbic acid in metabolic techniques is not so well

understood. There is certainly some proof that it may be engaged in metabolic hydroxylation

reactions of tyrosine, proline, and some steroid hormones, in addition to the cleavage-oxidation

of homogentisic acid. Its function in these metabolic processes definitely seems to be related to

the ability of vitamin C to act as a reducing agent.

The adult Recommended Daily Allocated f nutritional C is usually 70 magnesium per day. Some

scientists and medical doctors have recommended doses about 1 to 3 grams each day in order to help

withstand the common chilly. Deficiency of supplement C leads to swollen joint, abnormal

development and maintenance of tissues structures, and finally scurvy.

Determination of nutritional C in biological essential fluids such as bolld and urine is tough

because only small amounts from the vitamin exist and many interfering reducing

agents exist. Substances that contain sulfhydryl groups, sulfite, and thisosufate are

prevalent in biological fluids and react with DCIP, but much more slowly and gradually than ascorbic

acid solution. The disturbance by sulfhydryl is often reduced by the addition of

p-cholormercuribenzoic acidity.

Components and Items

Apple Juice

Metaphosphoric acid/ Lactic acid solution 4%

Unknown ascorbic acid in metaphosphoric acid/acetic acid option,. 5mg/ml a couple of, 6 dicholorphenolindophenol solution in water

Ascorbic acid oxidase, lyophilized normal water

Experimental Procedure

Standard Ascorbic Acid Option

Fill a microburet with DCIP option. Using a pipet, transfer 1 . 0 ml of the ascorbic acid

standard answer to a 55 ml Erlenmeyer flask containing 5 milliliters of 4% metaphosphoric acid solution

remedy. Read and record the first reading for the buret. Titrate by speedy, dropwise

addition of DCIP from your buret although mixing the contents in the flask. Add DCIP

solution right up until a distinct rose-pink color continues for 15 seconds. Record the final

reading within the buret. Continue this procedure 2 times more, everytime with a clean 1 . 0 ml

sample of ascorbic acid solution standard. In a similar vogue, titrate 3 blanks, every single

that contains 5. um ml of 4% metaphosphoric acid and 1 . zero ml of water. Typical the results

for each and every series of measurement. Do the same for the apple juice sample, and the two


# of TrialsStandardsBlank Unidentified #3Unknown #4Apple Juice 13. 9. twenty four. 63. 02. 9

twenty four. 0. twenty four. 42. 92. 7

33. 8. 24. 43. doze. 9

Average3. 9. twenty-four. 473. 02. 83


Apple Juice

The daily intake of vitamin C should be 70 mg

The Apple juice business claims to contain forty percent of the daily vitamin C intake. 40% (70 mg) = twenty-eight mg in 237 ml

10 cubic centimeters of the apple juice is diluted with water to 60 ml

28 mg (10 ml/237 ml) = 1 . 18 mg in 10 cubic centimeters

Theoretical quantities: 1 . 18 mg (10 ml/50 ml) =. 236 mg of vitamin C in the juice Genuine amounts:

2 . 83 (juice average) -. two (blank average) = installment payments on your 63

three or more. 9 (standard average) --. 2 write off average) sama dengan 3. several

( installment payments on your 63 ml/3. 7 ml). 5 mg =. 355 mg of Vitamin C

. 355 mg(10 ml/50 ml) sama dengan. 071 magnesium in 12 ml

(. 071 mg/10 ml)(237 ml) = 1 . 94 mg

1 . 94/70 (100) = installment payments on your 8% of the daily advised allowance pertaining to vitamin C

Unidentified #3

4. forty seven (average)-. 2(average blank) sama dengan (4. 27/3. 7)(. a few mg) =. 577 magnesium of...